Identification . Glycerol (C3H8O3), a sugar alcohol, is a simple . 14th Jan, 2022. 1) Mix 100 mg of bromophenol blue with 10 ml of ddH2O and mix. The investigation of a preferential solvation model that considers the addition of small quantities of alcohol to water in the presence of bromophenol blue (BPB) is described in this . 1.1. Reaction of bromophenol blue with water Bromophenol blue is tetrabromophenolsulfonphthalein, a natural dye, also used in laboratory practice as a pH indic. The pKa is about 4. Hexagonal crystals. Bromophenol Blue 95%: ECSA Chemicals: BROMOPHENOL BLUE SOLUTION 0.4% IN ETHANOL RP: ECSA TRADE THE MOST UPDATED FINANCIAL PUBLICATION ON THE WORLD OF CHEMISTRY: . USP, Ph. Solubility: Soluble in water. Dissolve 0.5 g of bromothymol blue in 500 mL of water. Slightly soluble in water. 77, No. Concentration: 0.2% Aqueous Composition: Water 99.80%, Bromophenol Blue, Sodium Salt 0.20% Density: 1 Color: Red to reddish-blue liquid Physical State: Liquid pH Range: 3.0 (yellow) - 4.6 (blue) Solubility Information: Miscible Shelf Life: 24 Months Storage: Ambient Chemical: 500mL PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing. Log Pow : No data available Auto-ignition temperature : No data available Bromophenol Blue, 0.1% Aqueous Safety Data Sheet Notes To 0.05 ml of the bromophenol blue solution add 20 ml of carbon dioxide-free water R and 0.05 ml of 0.1 M hydrochloric acid. cold water: soluble (Soluble in cold water and hot water) Description General description PlusOne Bromophenol Blue is an electrophoresis tracking dye used in IEF, PAGE and Sequencing. Policies. for analysis, ACS Quality Name: for analysis, ACS Headline Comment: pH indicator 3,0 greenish yellow; 4,6 blue Specifications: Identity: IR passes test Range of Transition interval according to . Auto-ignition temperature. USP, Ph. Persistence and DegradabilitySoluble in water Persistence is unlikely based on information available. Partition coefficient n-octanol/water (Log Pow) : No data available Buy Bromophenol blue (BPB)- an affordable, . Instituto Federal do Paran - IFPR, Lacoppi - Laboratrio de Corantes e Processos Pirolticos, Departamento de Qumica, Palmas, PR 85 . Dangui AZ et al (2018) Spectrochim Acta A Mol Biomol Spectrosc 203 : 333-341. Read more (PubMedID: 29885632) Bromophenol blue is also used as a pH indicator with a transition range of pH 3 to 4.6. Component Compounds: CID 112888. Bromophenol Blue, Water Soluble 34725-61-6 None None None None Australia and Mexico Components CAS-No. Ph. . National Center for Biotechnology Information. Bromophenol blue is an intermediate and Bromophenol Blue is used as an acid-based indicator in the pH range of 3-4.6 where the color changes from yellow to blue. Not for drug, food or household use. Dangui AZ 1, Santos VMS 1, Gomes BS 1, de Castilho TS 1, Nicolini KP 1, Nicolini J 1. Bromophenol Blue, Water Soluble is used as an acid-base indicator and a gel electrophoresis color marker. Shop Bromophenol Blue (Free Acid), Fisher BioReagents at Fishersci.ca Solubility in Water: No data available: Upper Explosion Limit: No data available: Vapor Pressure: No data available: If breathing is difficult, give oxygen. Bromophenol Blue, Sodium Salt, ACS (62625-28-9) Not applicable Water (7732-18-5) Not applicable 8.2. If not breathing, give artificial respiration. Synonyms and Related Terms. Solubility: water 3 g/l at 20 C alcohol 9 g/l Physical Description: solid Product Code: 131165 Product Name: Bromophenol Blue (Reag. Freely soluble in NaOH Alfa Aesar 32641: Soluble to 10 mM in ethanol Tocris Bioscience 5218: Experimental Density: 105 g/mL Biosynth J-610052: Soluble in ethanol, ether, ethyl acetate, benzene, alkali solutions. Molecular Formula C 19 H 10 Br 4 O 5 S; Average mass 669.961 Da; Monoisotopic mass 665.698242 Da; ChemSpider ID 7973; More details: Systematic name. 2022-04-02. (11) Run gels at 20 mA, constant current, with anode (positive) at bottom, until bromophenol blue line reaches bottom . Use process enclosures, local exhaust ventilation, or other . Bromophenol blue Hayashi Pure Chemical Ind.,Ltd. Solubility: Soluble in water (30 mg/ml-clear, blueish violet solution) or ethanol (7 mg/ml) . Composition C19H10Br4O5S CAS 115-39-9 Melting Point 273 C Molecular Weight BROMOPHENOL BLUE - Bromophenol Blue Indicator Revision nr.1 Dated 11/01/2017 Printed on 12/01/2017 Page n. 1 / 7 EN EPY 9.3.0 - SDS 1003 Information Sheet . The dye is not very soluble. Department of Health and Human Services. 77, No. In solution, bromophenol blue gives a yellow color below pH 3.0 and purple color above pH 4.6. Shake well and observe the color of the mixture. Herein, we introduce a new approach for the determination of acid dissociation constant based on digital image analysis using a low-cost, precise, accurate, sensitive, and portable home-made . Bromophenol blue is available commercially as a light pink to purple crystalline powder which is readily soluble in water (solubility in water is ~ 1 mg/ml) and other organic solvents e.g., ethanol, DMSO, and dimethyl formamide (DMF). for analysis, ACS Quality Name: for analysis, ACS Headline Comment: pH indicator 3,0 greenish yellow; 4,6 blue Specifications: Identity: IR passes test Range of Transition interval according to ACS: passes test T.L.C. Bromophenol Blue Indicator Solution; Strong Bromophenol Blue Indicator Solution; Ethanolic Bromophenol Blue Indicator Solution: Dissolve 0.1 g of bromophenol blue with gentle heating in 1.5 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent) and add sufficient water to produce 100 ml. National Institutes of Health. Ideal tracking dye for nucleic acid gel electrophoresis. Product form : Substance Substance name : Bromophenol Blue, Sodium Salt . Create . It is even used as a color indicator, acid-base pH indicator and as a biological stain. Please enter your email id and click update. . Not available. Bromophenol blue is used as a tracking dye for electrophoresis. The SEM analysis . Inhalation Move casualty to fresh air and keep at rest. Eur.) Heat sample in sample buffer in boiling water bath for 2-5 min. Safety Information Storage Class Code 13 - Non Combustible Solids SAFETY DATA SHEET: BROMOPHENOL BLUE, Indicator Solutions BROMOPHENOL BLUE, Indicator Solutions B809.7E; B809.7M INTEGRA Chemical Company 24 Hour Emergency Response: CHEMTREC 800-424-9300 (Outside USA 703-527-3887) 1216 6th Ave N Kent WA 98032 Phone: 253-479-7000 Mixture NA Recommended Use: Restrictions on Use: Commercial/industrial use Product . Bromophenol blue is an acid phthalein dye. Yellow liquid Solubility/miscibility Soluble in ethanol, methanol, benzene, and sodium hydroxide solutions. Acid: yellow, Base: blue. . 115-39-9 . That is, it is useful as a pH indicator. Pigment & Dyestuff; Organic Intermediate; Organic chemical; Flavour & Fragrance ; Health & Medical; Chemical reagent; Catalysts & Chemical Auxiliary Agents . Cite. Exposure controls Appropriate engineering controls : Emergency eye wash fountains should be available in the immediate vicinity of any potential exposure. It changes from yellow at pH 3.0 to purple at pH 4.6 and this reaction is reversible. Chemsrc provides Bromophenol Blue(CAS#:115-39-9) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. Synthesis and preparation BROMOPHENOL BLUE: PRODUCT IDENTIFICATION . Not available. The color of aqueous solution of bromophenol blue is pH dependent. Cite. But your solution will be really blue. I'm trying to determine if powdered Bromophenol blue is safe to mix with Pentane. Customer Service (714) 754-1339 My Cart $ 0.00. At pH 3 it will give a yellow color and pH above 4.8 it will give a blue color. Name: bromophenol blue Solubility Freely soluble in NaOH. Eur.) Eur., CAS Number: 115-39-9,(32712 Fluka) . Bromophenol blue is also used as a dye. Solubility: water 3 g/l at 20 C alcohol 9 g/l Physical Description: solid Product Code: 131165 Product Name: Bromophenol Blue (Reag. Most important symptoms and effects, both acute and delayed . Soluble in ethanol. EINECS NO. Preferential solvation bromophenol blue in water-alcohol binary mixture. Not Intended for Diagnostic or Therapeutic Use. Bromophenol blue is a pH indicator. The invention relates to a preparation method of bromophenol blue, which comprises the following steps: dissolving 1kg of phenol red in 3L of glacial acetic acid; dissolving 0.64L of bromine in 0.8L of the glacial acetic acid to get bromine diluent; heating phenol red solution to 60 DEG C under a stirring condition, slowly dropwise adding the bromine diluent, continuously heating up to 100-105 . At low pH, the dye absorbs ultraviolet and blue light most strongly and appears yellow in solution. More soluble in methyl and ethyl alcohols ,benzene, and in acetic acid. The pK is 4.0. Bromophenol Blue. Bromophenol blue water solubule. Preferential solvation bromophenol blue in water-alcohol binary mixture. Mixture Bromophenol blue, sodium hydroxide and water Atomic weight 669.99 Weight per litre Solution: 0.96kg Appearance Light orange powder. * In solvent : -80C, 6 months; -20C, 1 month (sealed storage, away from moisture and light) mouth with water. Spectrochim Acta A Mol Biomol Spectrosc. Intended Use of the Product Use of the Substance/Mixture: For laboratory use only. Chemsrc provides Bromophenol Blue sodium salt(CAS#:62625-28-9) MSDS, density, melting point, boiling point, structure, formula, molecular weight etc. = 0.010 grams of Bromophenol blue in 20 mL of water makes 0.05% (mass/volume) bromophenol blue. The bromophenol blue It is a chemical substance of organic nature, which due to its property of turning under certain pH values is used to titrate chemical substances. Reliable Water Content Determination by Karl Fischer Titration with NEXTGEN Smart Chemicals . 669.96. Box 159, 37888 . Apply artificial respiration if not breathing. It was used in serum albumin determinations. Both of free acid and sodium salt are used as pH indicator. The reaction is reversible. 1 Structures Expand this section . (10) Overlay sample with running buffer. Bromophenol blue sodium salt is a tracking dye for protein and nucleic acid electrophoreses: CAS Number: 34725-61-6: Molecular Weight: 691.94: Molecular Formula: . Skin ContactWash off immediately with plenty of water for at least 15 minutes. Solubility: water 3 g/l at 20 C alcohol 9 g/l Physical Description: solid Product Code: 131165 Product Name: Bromophenol Blue (Reag. Molecular Weight. Availability: Catalog Number Description Size 150522 Bromophenol Blue, Sodium Salt 5 g 10 g 25 g The composition of agarose gel defines the moving position of bromophenol blue in the gel. Bromophenol Blue, sodium salt Revision Date 24-Dec-2021 12. PubChem CID: 101059631: Structure: Find Similar Structures. Quotation $ 0.00 Solubility : Sparingly soluble in water. for analysis, ACS Quality Name: for analysis, ACS Headline Comment: pH indicator 3,0 greenish yellow; 4,6 blue Specifications: Identity: IR passes test Range of Transition interval according to . (yellow) and 5.4 (blue). It is a brown powder sparingly soluble in water, soluble in benzene, and very soluble in ethanol and diethyl ether. USP, Ph. The protonated form of bromophenol blue is badly soluble in water. First-aid measures If inhaled, remove from contaminated area to fresh air immediately. . Bromophenol blue is soluble in water (solubility in water is ~ 1 mg/ml) and carries net negative charge at neutral or slightly basic pH (the pH of the electrophoresis buffer). An Email Id is required to process your orders. Solubility soluble in water Partition coefficient: n-octanol/water Not available. Molecular Formula: C 19 H 9 Br 4 O 5 SNa. Author information. At neutral pH, the dye absorbs red light most strongly and transmits blue light. In this study, the perichromic behavior of bromophenol blue (BPB) in various binary solvent mixtures was investigated. Solubility: Soluble in cold water and hot water : Physical State: Solid : Color: Purple, black : Storage conditions: Room Temperature : Adjust pH to > 5-6 by NaOH or use a buffer with pH >6 Cite 11th Feb, 2020 Ramyani De Georgia State University. The bromophenol blue co-migrates with ~300 bp DNA fragments in 1% agarose gel. 8600 Rockville Pike, Bethesda, MD, 20894 USA. Product Name: BROMOPHENOL BLUE, 0.04% AQUEOUS SOLUTION Synonyms: Bromophenol Blue, Water Solution 1.2. First-aid measures Eye ContactRinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. 77, No. Analysis Note To view the Certificate of Analysis for this product, please visit www.cytiva.com. Solubility : Soluble in water. Bromophenol Blue Indicator, Reag. Molecular Formula: C 19 H 10 Br 4 O 5 S. For Research Use Only. 58 / Monday, March 26, 2012 / Rules and Regulations Date of issue: 01/31/2014 Revision date: 12/19/2016 Supersedes: 05/13/2015 Version: 1.2 12/19/2016 EN (English US) Page 1 SECTION 1: Identification . Item Value; Product Name : bromophenol blue. Soluble in methanol (10 mg/mL.). Solutions of the dye, therefore, are blue. Affiliations. If breathing is difficult, give oxygen . Preferential solvation bromophenol blue in water-alcohol binary mixture In this study, the perichromic behavior of bromophenol blue (BPB) in various binary solvent mixtures was investigated. Bromophenol blue solution 1012801. Reagents. CID 5360545 (Sodium) Dates: Modify . Name, Address, and Telephone of the Responsible Party Manufacturer Quality Science Labs, LLC P.O. Molecular Weight: 669.96. However, there are other substances that serve as solvents such as acetic acid, methyl alcohol . Molecular Weight: 691.94. Molecular Formula: C 19 H 9 Br 4 NaO 5 S: Synonyms: Bromophenol blue water solubule. Bioaccumulation/ AccumulationNo information available. The binary mixtures considered were comprised of water and methanol (MeOH), ethanol (EtOH), n-propanol (n-PrOH), isopropanol (iso-PrOH) or t-butanol (t-BuOH).The investigation of a preferential solvation model that considers the addition of small quantities of alcohol to water . Eur.) Concentration: 0.2% Aqueous Composition: Water 99.80%, Bromophenol Blue, Sodium Salt 0.20% Density: 1 Color: Red to reddish-blue liquid Physical State: Liquid pH Range: 3.0 (yellow) - 4.6 (blue) Solubility Information: Miscible Shelf Life: 24 Months Storage: Ambient Chemical: 500mL . CAS Number: 62625-28-9 Formula Weight: 691.97 Formula: C 19 H 9 Br 4 NaO 5 S Density (g/mL): >1 Boiling Point (C): Decomposes Freezing Point (C): Decomposes Solubility: Water Synonyms: Bromophenol Blue, pH Indicator Shelf Life (months): 36 Storage: GreenChemicals for science education are available in easy-to-use formats with instructions for students to be introduced to a variety of subjects. 5.6.3 Prepare bromothymol blue acid-base indicator. 4.2. Water solubility : No data available Partition coefficient: n-octanol/water : No data available . It is a weak acid and available as a light pink to a purple crystal and water-soluble. Parent Compound: CID 112888. Ecological information Ecotoxicity Do not empty into drains. Application: Bromophenol blue sodium salt is a tracking dye for protein and nucleic acid electrophoreses. PlusOne Bromophenol Blue. Get medical attention. Related Products: Bromophenol Blue Indicator. Bromophenol blue is also used as a pH indicator, with a transition range of pH 3 to 4.6 [1] [2] . since it is slightly soluble in water. Mobility Will likely be mobile in the environment due to its water solubility. Contact. 204-086-2 . It is less soluble in nonpolar solvents such as benzene, toluene, and xylene, and practically insoluble in petroleum ether. Date of issue: 6/9/2010 Revision date: 5/8/2020 SDS code: A5-04 Version: 04.1 1/5 . More soluble in methy and ethyl alcohols ,benzene, and in acetic acid.Slightly soluble in water (0.4g/100g) at 20 C. Preferential solvation bromophenol blue in water-alcohol binary mixture. Storage & Solubility Information. Weight 669.96 Uses of Bromophenol Blue: It is used as an acid-base indicator its useful range lies between pH 3.0 and 4.6. Add a drop of ammonia solution to turn the solution deep blue in colour. Bromphenol blue 115-39-9 >95 4. At pH 3.0 it is blue and at pH 4.6 it changes to yellow. CAS NO. Product Image : Cas Number : 115-39-9. Main page (Russian) Search in database (English) Sitemap (Russian) Properties of substance: bromophenol blue Synonyms: 3,3',5,5'-tetrabromophenolsulfonephthalein . Bromophenol blue solution: Add 20 ml of 0.1% bromophenol blue in 96% ethanol to a mixture of 75 ml 0.2 N sodium acetate and 925 ml 0.2 N acetic acid. Dangui AZ, et, al. As an acid-base indicator its useful range lies between pH 3.0 and 4.6. Jinan Boss Chemical Industry Co.,Ltd +86 531 68657995 +86 13280017993; [email protected]; HOME; Products. Bromophenol Blue, 0.1% Aqueous Safety Data Sheet according to Federal Register / Vol. Is bromophenol blue soluble in water? Not for drug, food or household use. Bromothymol blue is sparingly soluble in oil, but soluble in water, ether, and aqueous solutions of alkalis. Articles of Bromophenol Blue are included as well. Length of sample must be less than length of stacking gel, as measured from bottom of well to main gel. (9) Load sample. FOIA. 2018 Oct 5; 203: 333 . Bromophenol Blue, Sodium Salt, ACS Safety Data Sheet according to Federal Register / Vol. PubMed:The flagellar membrane of Ochromonas danica. Decomposition temperature. 6 authors. CAS Number: 115-39-9. Bromophenol Blue 100 62625-28-9 263-653-2 C 19 H 9 Br 4 NaO 5 S 691.94 g/mol 4. Bromophenol Blue, Water Soluble, ACS, Spectrum Chemical Click to view available options Quantity: 5 g 25 g 100 g 1 kg Packaging: Amber Glass Bottle CAS: 34725-61-6: CAS: 34725-61-6: Description . The binary mixtures considered were comprised of water and methanol (MeOH), ethanol (EtOH), n -propanol ( n -PrOH), isopropanol (iso-PrOH) or t -butanol ( t-BuOH). Formula. Bromophenol blue 115-39-9 100 % Commonly used as the water-soluble sodium salt [62625-28-9] or as solution in dilute sodium hydroxide or alcohol/water. The NaTNT powder was mixed with car's tire powder (TP) to enhance its light absorption or was supported on recycled car's air filters (AFs) to facilitate its removal from the cleaned water after the degradation of the BPB. 3.0 - 4.6 . Solution miscible with water Melting point 273C with decomposition Boiling point Decomposes Other Information 4. Thermo Scientific Bromophenol Blue, sodium salt, pure, water soluble, indicator 25g, Glass bottle, Each Quantity Packaging Glass bottle Specification Sheet Certificates SDS Catalog number: 151350250 Price (USD) Contact Us Request bulk or custom quote Save to list Technical Support Customer Service Specifications Specification Sheet